The purpose of the study was designed to find out the effect of six weeks zinc supplementation on selected physical, physiological and hematological variables among basketball players. In this study, the investigator selected 20 male basketball players from Shanmuganathan Engineering College, Arasampatti, Pudukkottai, Tamil Nadu, India. Their age were ranged between 18 to22 years. These players were randomly distributed into two groups namely experimental (10) and control (10) group respectively. The Independent variable selected for the present study was supplementation of zinc. The criterion variables selected for this study were Physical (Speed & Power), Physiological (VO2max & Blood pressure) and Hematological (Neutrophils, Lymphocytes and Eosinophil). The data were statistically examined by applying one way univariate ANCOVA from the collected data before and after the experimental period. The result of the study reveals that there existed significant improvement on VO2max, Neutrophils, Lymphocytes & Eosinophil and insignificant improvement on Speed, Power and Blood Pressure.