This research was aimed to determine the effects of eight weeks pre-season preparation training period on the physical and physiological characteristics of football players. Thirty football players were acted as subjects for this study. Their ages were 19.5 ± 1.46 years old, and they had 4.3 ± 1.2 years of training. Their height was 167.7 ± 4.12 cm. The body weight, body fat percentage, flexibility, systolic/diastolic blood pressure, aerobic capacity, vertical jump, and speed of these players were tested twice; once at the beginning of the eight-week pre-season preparation training period and again at the end of the training period. Research data was evaluated statistically with paired-t test at a significance level of (p‹ 0.05). Significant changes were found in weight, body fat percent, systolic/diastolic blood pressure, aerobic capacity, anaerobic power, and vertical jump but no significant changes were found in elasticity and speed.