The aim of the study was to compare the stress level among the national level female and male handball players. For the purpose of the study a sample of 60 players (30 male and 30 females) by random sampling was selected at All-India Inter-University Competition at Panjab University, Chandigarh in the year 2012. Stress Inventory developed and standardized by A.K.Singh (2004) was used as a tool for the data collection. The researcher has compared the means of two groups by employing independent t-test. The results have shown that the mean value of females on stress level as compared to the males was higher.