The aim of this study was to compare the maximum voluntary ventilation (MVV) between smokers and non-smokers of 60 male university students (30 each in both groups). The age of the subjects ranged between 21 – 26 years. The subjects were purposely selected from ITM University, Sithaouli Road, Gwalior (M.P), India. Spiro-metric test (A Spiro-meter connected with a computer- Minispir) was conducted on them considering the parameter MVV. It was measured through Spiro-meter in liter per minute. For the data analysis t-test was employed on the data of smokers and non-smoker groups and the level of significance was set at 0.05. Comparative study of maximum voluntary ventilation between smokers and non-smokers concluded that non-smokers have greater maximum voluntary ventilation in comparison with smokers.