The purpose of this study was to compare the vital capacity and peak flow rate among different playing positions in basketball i.e. guards, forwards and centers. To achieve the purpose of the study forty five male basketball players from four different universities of Madhya Pradesh who have participated in west zone Inter University basketball competition were selected. They were then divided into three different subsamples according to their positional role (guards = 22, forwards = 19 and centers = 14). To measure the vital capacity and peak flow dry Spirometer and peak flow meter were used respectively. To test the significance of the mean difference among the players of various positions namely guard, forward and center, one way analysis of variance was used. As post – hoc test, the Schefee’s test was applied. The results revealed that the forwards ( 540.62 & +62.55) demonstrated the highest peak flow rate than the guards ( 474.28 & +46.19). Further there were no significant differences found in vital capacity among different playing positions in basketball i.e. guards, forwards and centers.