This researcher work discussed about the origin of related problem. 140 Teachers from Teacher Training Institute’s of Gujarati medium Teacher Training Institutes of urban and rural areas of Ahmedabad district were selected as a sample for study. Multi-stage sampling technique was used for data collection. Survey method was used as research methodology. A standardized tool of Dr.D.I.Patel was administrating to measure the aptitude of Teacher Training Institute’s Teachers of Gujarat state. Mean, standard deviation and t-test were used for analysis and interpretation of data. Thus the whole overview of research design was presented in a systematic manner.The researcher did the analysis and interpretation of data by using various statistical methods like mean, standard deviation and t-test. On the basis of derived results, the researcher interpreted whether the framed null hypothesis was rejected or not rejected. Thus the researcher found out the professional effectiveness of Teacher Training Institute’s Teachers of Ahmedabad district.The researcher discussed about the findings, Educational implementation in detail.