A longer relative fourth digit to second digit is indicative of increased intrauterine testosterone exposure prenatally and the converse is also true for estrogen exposure. But there are very few studies which has evaluated the association between 2D:4D and the testosterone hormone in saliva of elite sportsmen. The aim of this study was to test the hypothesis that high levels of testosterone during prenatal life, testified by a low second-to-fourth digit ratio (2D:4D), as well as in adulthood affect the salivary testosterone in the elite players. Digital finger ratio 2d:4 d and salivary testosterone hormones was measured in 91 elite sportsmen (Volleyball N-36, Basketball N- 30 & Football N- 25) of age 22.14 ±2.11 yrs. We found a significant difference in the digital finger ratio 2d:4d between the elite sportsmen and non-sportsmen (Volleyball v/s NSM t value -3.89, P < 0.01, Basketball v/s NSM t value – 3.01, P < 0.01 & Football v/s NSM t value – 4.33, P < 0.01). Further significant difference (Volleyball v/s NSM t value -7.29, P < 0.01, Basketball v/s NSM t value – 4.10, P < 0.01 & Football v/s NSM t value – 8.57, P < 0.01) in the level of salivary testosterone hormone (pg/ml) between the elite sportsmen and non-sportsmen was found. We suggest that low right-left 2D:4D is a predictive marker of testosterone receptiveness in men trained for sports and this association is programmed by the action of prenatal testosterone.